Objective To evaluate the patients’ and hospital staff’s respective perception of medical service quality for assessment of the patients’ satisfaction with the medical service as well as the staff’s satisfaction with their work，so as to improve the quality of the medical service provided by the hospital. Methods Two types of scales (one for patients and one for staff) were designed according to the five ServQual dimensions. Each scale contained 22 questions. These questionnaires were completed by randomly selected outpatients, inpatients and hospital staff. The hospital staff was also asked to complete the questionnaire of self-evaluation of the medical service they provided for the patients. The SPSS20.0 statistical software was used for data analysis. Results The patient questionnaires as well as staff self-evaluation questionnaires indicated high satisfaction regarding all the five dimensions based on the perceived service quality. The staff questionnaires indicated that the perceived service quality was satisfactory regarding tangibles，less satisfactory regarding assurance and responsiveness，and unsatisfactory regarding empathy and reliability. The female staff had higher level of satisfaction than male staff based on their respectively perceived service quality. The perceived service quality was unsatisfactory in the staff of the age group of 30～49 years，and also in the clinical staff，who are in the first line of medical service，especially the medical technicians and doctors. Conclusion The hospital should guarantee its staff’s legal rights and deserved benefits，and make sure that the working period as well as working intensity is appropriate. The improvement of staff-perceived medical service quality is a prerequisite for the improvement of patient-perceived medical service quality.